| 產品編號 | BIO0988SM |
| 英文名稱 | Anti-4-1BB & HER2/neu Reference Antibody (Yh32367 Biosimilar) |
| 別 名 | TNFRSF9 / 4-1BB / CD137 & ERBB2 / HER2 / CD340; Yh32367 |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 交叉反應 | Human |
| 產品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 200.52kDa |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-ScFv |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 | |
| 產品圖片 |
Co-incubation of Yh32367 with BT474 cell and PBMCs for 4 hours, then LDH was detected to evaluate the ADCC activity of Yh32367. As shown in fig, Yh32367 has ADCC activity, and the IC50 was 0.054 nM.
Yh32367 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to hu4-1BB-CHO-K cells, and the EC50 was 5.701 nM.
Yh32367 bound to hu4-1BB-CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to hu4-1BB-CHO-K cells, and the EC50 was 5.701 nM.
Yh32367 bound to BT474 cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Yh32367 bound to BT474 cells, and the EC50 was 1.889 nM.
Yh32367 bound to Her2 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-Her2-His, and the EC50 was 0.009 nM.
Yh32367 bound to 4-1BB protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Yh32367 bound to hu-4-1BB-His, and the EC50 was 0.007 nM.
The purity of Anti-4-1BB & HER2/neu Reference Antibody (Yh32367) is 98.99%, determined by SEC-HPLC.
Anti-4-1BB & HER2/neu Reference Antibody (Yh32367) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
