| 產品編號 | bsm-60432R |
| 英文名稱 | TSHB Recombinant Rabbit mAb |
| 中文名稱 | 促甲狀腺素β亞單位重組兔單抗 |
| 別 名 | TSHB_HUMAN; Thyrotropin subunit beta; Thyroid-stimulating hormone subunit beta(TSH-B; TSH-beta); Thyrotropin beta chain; Thyrotropin alfa; TSH-β; |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | D7C6 |
| 交叉反應 | Rat |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The four human glycoprotein hormones chorionic gonadotropin (CG), luteinizing hormone (LH), follicle stimulating hormone (FSH), and thyroid stimulating hormone (TSH) are dimers consisting of alpha and beta subunits that are associated noncovalently. The alpha subunits of these hormones are identical, however, their beta chains are unique and confer biological specificity. Thyroid stimulating hormone functions in the control of thyroid structure and metabolism. The protein encoded by this gene is the beta subunit of thyroid stimulating hormone. Mutations in this gene are associated with congenital central and secondary hypothyroidism and Hashimoto's thyroiditis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Subcellular Location: Secreted. Database links: Entrez Gene: 7252 Human
SwissProt: P01222 Human TSHB（甲狀腺刺激激素亞基Beta）是一種蛋白質編碼基因。 與TSHB相關的疾病包括甲狀腺功能減退，先天性，非甲狀腺功能減退4和中樞性先天性甲狀腺功能減退。其相關途徑包括通過GPCR進行信號傳遞和肽激素代謝。與該基因相關的基因本體論（GO）注釋包括激素活性。該基因的重要旁系同源物是FSHB。 |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with TSHB monoclonal antibody, unconjugated (bsm-60432R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Rat pituitary; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSHB Monoclonal Antibody, Unconjugated (bsm-60432R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
